This is the first image I processed using Shinestacker. It's a remarkable and technically advanced piece of open source software. This software is beyond my skill level, but works incredibly well with defaults.
Sufficeth to say it was very simple, after a somewhat convoluted---but not difficult---installation on CachyOS, to process this stack of 40 images. I have a CPU with 27 or 28 processors; and this software even took advantage of my aging Nvidia GTX-1050 video card with a mere 3GB of video ram. 25X objective, mounted in UV-curing glass glue from a hardware store. Too bad about the detritus. Processing took a few seconds.
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| Ossicles of Holothuria atra mounted in UV-curing glass glue. |

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